ContactCRYOTOP®
Not just the best proven results on the market, now also the best user experience.
Achieve the highest survival rates on the market with the Cryotop® Method, the global leader in vitrification of oocytes and embryos in all stages of development.
The revolutionary device by Kitazato has been applied in over 1,500,000 clinical cases for oocytes and embryos with this method of cryopreservation during the last decade, obtaining excellent results in over 90 countries and 2,200 assisted reproduction centres. The Cryotop® method is simple, safe, universal and easy to apply for everyone.
CRYOTOP® IMPROVEMENTS
Main improvements:
Wider Strip: The strip’s been widened. Now, with a bigger surface, it is even easier to load the specimens.
Easier Insertion: The diamond tip, the reshaped conjunction between strip and handle and the widened body grant a smoother insertion into the coverstraw.
Identification Marks: The Cryotop® has now two identification marks on its upper surface, defining the side where the specimens should be loaded, if you are vitrifing, or are loaded, if you are thawing.
Cryotop® is a vitrification device consisting of a fine strip of transparent film attached to a plastic handle resistent to liquid nitrogen. Its design allows the loading of specimens for vitrification with a minimum volume (0.1 µl), providing the best Cooling and Warming rates on the market (-23,000ºC/minute and 42,000ºC/minute respectively) which in turn lead to the best survival rates.
Highest survival rates
Cryotop® Method offers proof of its outstanding results. Our suvival rates over 90% are backed up by numerous clinical studies with the biggest sample sizes in the market.
- 100%
- 90%
- 80%
- 70%
- 60%
- 50%
- 40%
- 30%
- 20%
- 10%
- 0%
- Oocytesn=3.286
Cobo
2010 - Oocytesn=124
Rienzi
2010 - Oocytesn=168
Parmegiani
2011 - Oocytesn=898
Solé
2013 - Embryosn=124
Rienzi
2012 - Embryosn=168
Parmegiani
2012 - Embryosn=898
Solé
2013 - Blastocystsn=168
Parmegiani
2011 - Blastocystsn=898
Solé
2012
Cryotop® Survival rates in human specimen
Main characteristics:
- Allows up to 4 specimens to be loaded per device.
- It has an external covering to protect the Cryotop® once vitrified. Tip with weight to prevent floating in liquid nitrogen.
- Manufactured in malleable plastic resistant to liquid nitrogen.
- Available in packs of 10 units.
Cryotop® is the logical choice for obtaining the best clinical results. Its unparalleled survival rates for oocytes and embryos have contributed to bringing hundreds of healthy babies into the world in the last decade.
Accessories
Repro plate
Exclusively designed to follow the vitrification protocol with comfort; offers two slots to support the Cryotop®, allowing those who wish to carry out loading specimens statically. Has a flat base which allows the use of traceability labels.
Cooling rack
Designed to contain the liquid nitrogen during vitrification. Metallic cover for the interior also available, allowing sterilisation before use.
| ORDER NUMBER | CODE | DESCRIPTION |
| 81115 | CR-Y | Cryotop® Yellow |
| 81114 | CR-B | Cryotop® Blue |
| 81113 | CR-W | Cryotop® White |
| 81112 | CR-R | Cryotop® Red |
| 81111 | CR-G | Cryotop® Green |
| K | Reproplate | |
| 94123 | SET RACK-L | Cooling Rack+Steel Box-Large |
| 94122 | SET RACK-S | Cooling Rack+Steel Box-Short |
| 91101 | VT601 | Vitrification Solutions * |
| 91121 | VT602 | Thawing Solutions * |
| 91171 | VT801 | Vitrification Solutions |
| 91182 | VT802 | Thawing Solutions |
| 81125 | CR SC-Y | Cryotop® SC Yellow |
| 81124 | CR SC-B | Cryotop® SC Blue |
| 81123 | CR SC-W | Cryotop® SC White |
| 81122 | CR SC-R | Cryotop® SC Red |
| 81121 | CR SC-G | Cryotop® SC Green |
| 84115 | BLOCK SC | Aluminum Block for Cryotop® SC |
| 84117 | CUTTER SC | Straw Cutter for Cryotop® SC |
| 84119 | HEAT SEALER | Heat Sealer for Cryotop® SC |
- Endotoxin: ≤ 0.5EU/device by LAL methodology
- Sterility (Bacteria, Fungi): current USP Sterility Test <71>
- MEA (Mouse Embryo Assay): One cell assay ≥ 80% after 96 hours
Instructions
- Cobo A., Effect of oocyte vitrification on embryo quality: time-lapse analysis and morphokinetic evaluation. Fertility & Sterility, 2017.
- Diaz-Garcia C., Oocyte vitrification versus ovarian cortex transplantation in fertility preservation for adult women undergoing gonadotoxic treatments: a prospective cohort study. Fertility & Sterility, 2018.
- Coello A., Effect of oocyte morphology on post-warming survival and embryo development in vitrified autologous oocytes. Reproductive Biomedicine Online, 2019
- Domingues TS., Egg donation of vitrified oocytes bank produces similar pregnancy rates by blastocyst transfer when compared to fresh cycle. Journal Assisted Reproduction Genetics, 2017.
- Rienzi L., Definition of a clinical strategy to enhance the efficacy, efficiency and safety of egg donation cycles with imported vitrified oocytes. Human Reproduction, 2020
- Cobo A., Outcomes of vitrified early cleavage-stage and bastocyst-stage embryos in a cryopreservation program: evaluation of 3,150 warming cycles. Fertility & Sterility, 2012.
- La Marca A., A novel transnational fresh oocyte donation (TOD) program based on transport of frozen sperm and embryos. Human Reproduction, 2019.
- Zhang J., The impact of embryo quality on singleton birthweight in vitrified-thawed single blastocyst transfer cycles. Human reproduction, 2020.
- Berkkanoglu M., Optimal embryo transfer strategy in poor response may include freeze-all. Journal Assisted Reproduction Genetics, 2017.
- Cardenas Arma D.F., Frozen-thawed blastocyst transfer in natural cycle increase implantation rates compared artificial cycle. Gynecological Endocrinology, 2019.
- Chamayou S., The Accumulation of Vitrified Oocytes Is a Strategy to Increase the Number of Euploid Available Blastocysts for Transfer After Preimplantation Genetic Testing. Journal Assisted Reproduction Genetics, 2017.
- Cimadomo D., Associations of blastocyst features, trophectoderm biopsy and other laboratory practice with post-warming behavior and implantation of blastocyst features, trophectoderm biopsy and other laboratory practice with post-warming behavior and implantation. Human Reproduction, 2018.
- Hernandez-Nieto C., What is the reproductive potential of day 7 euploid embryos?. Human Reproduction, 2019.
- Cobo A., Viral screening of spent culture media and liquid nitrogen samples of oocytes and embryos from hepatitis B, hepatitis C, and human immunodeficiency virus chronically infected women undergoing in vitro fertilization cycles. Fertility & Sterility, 2012.
- Ueno S., Cryostorage duration does not affect pregnancy and neonatal outcomes: a retrospective single-centre cohort study of vitrified–warmed blastocysts. Reproductive Biomedicine Online, 2018
- Costa-Borges N., Cryopreservation of oocytes and embryos in times of COVID-19: Can the cure be worse than the disease? Risk assessment in the IVF laboratory. Fertility and Sterility, 2020.
