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Vitrification and Warming Media

Kitazato media are the most versatile option for cryopreservation in your laboratory. Reduce your costs by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from zygote stage to blastocyst.

Cryopreservation solution - Media for vitrification and warming of oocytes and embryos

The revolutionary device by Kitazato has been applied in over 1,500,000 clinical cases for oocytes and embryos with this method of cryopreservation during the last decade, obtaining excellent results in over 90 countries and 2,200 assisted reproduction centres. The Cryotop® method is simple, safe, universal and easy to apply for everyone.

VT801 Vitrification Media

The composition of the Kitazato media is entirely synthetic. Among the components, the following are notable:

  • Hydroxypropyl cellulose (HPC) prevents the risk of contamination, increases the rate of survival in hatched blastocysts and reduces mechanical stress during warming.
  • Trehalose functions as an osmotic agent in place of sucrose. It provides greater safety in the process, improving the protection of the cellular membranes.
  • The incorporation of gentamicin prolongs the sterility of the media, guaranteeing greater safety in handling the solutions.

The VT601 Vitrification media allow up to 5 procedures to be carried out and consist of:

  • 1.5 ml Basic Solution vial (BS)
  • 1.5 ml Equilibrated Solution vial (ES)
  • 2 x 1.5 ml Vitrification Solution vial (VS)


The VT602 Thawing media allow up to 4 procedures to be carried out and consist of:

  • 2 x 4 ml Thawing Solution vial (TS)
  • 4 ml Diluent Solution vial (DS)
  • 4 ml Washing Solution vial (WS)

All vitrification media must be kept between 2ºC and 8ºC.

VT601 and VT602 have a shelf life of 12 months.

VT801 and VT802 available in selected countries.

VT802 Thawing Media

Highest survival rates

Cryotop® Method offers excellent survival rates for oocytes and embryos in all stages of development, reported in numerous clinical publications with the largest study samples in the whole sector 

Cryotop® Survival rates in human specimen

  • 100%
  • 90%
  • 80%
  • 70%
  • 60%
  • 50%
  • 40%
  • 30%
  • 20%
  • 10%
  • 0%
  • Oocytesn=2.770
    Braga D.P.
  • Oocytesn=591
    Chamayou S.
  • Oocytesn=35.654
    Cornet-Bartolomé D.
  • Embryosn=303
    Xue Y.
  • Embryosn=2.617
    La Marca A.
  • Blastocystn=528
    Coello A.
  • Blastocystn=8.736
    Ueno S.
  • Blastocystn=3818
    Hernández-Nieto et al.
  • PGT Embryosn=90
    Sekhon L.
  • PGT Embryosn=2.129
    Cimadomo D.

In our publications section you will find the most relevant research on vitrification conducted using Kitazato products by IVF professionals and scientists from all over the world.

91101 VT601 Vitrification Solutions *
91121 VT602 Thawing Solutions *
91171 VT801 Vitrification Solutions *
91182 VT802 Thawing Solutions *
81115 CR-Y Cryotop® Yellow
81114 CR-B Cryotop® Blue
81113 CR-W Cryotop® White
81112 CR-R Cryotop® Red
81111 CR-G Cryotop® Green
K Reproplate
94123 SET RACK-L Cooling Rack+Steel Box-Large
94122 SET RACK-S Cooling Rack+Steel Box-Short
81125 CR SC-Y Cryotop® SC Yellow
81124 CR SC-B Cryotop® SC Blue
81123 CR SC-W Cryotop® SC White
81122 CR SC-R Cryotop® SC Red
81121 CR SC-G Cryotop® SC Green
84115 BLOCK SC Aluminum Block for Cryotop® SC
84117 CUTTER SC Straw Cutter for Cryotop® SC
84119 HEAT SEALER Heat Sealer for Cryotop® SC
*These products are only available in selected countries

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