Vitrification and Warming Media

Kitazato media are the most versatile option for cryopreservation in your laboratory. Reduce your costs by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from Pronucleus to Blastocyst.

Cryopreservation solution - Media for vitrification and warming of oocytes and embryos

The revolutionary device by Kitazato has been applied in over 1,500,000 clinical cases for oocytes and embryos with this method of cryopreservation during the last decade, obtaining excellent results in over 90 countries and 2,200 assisted reproduction centres. The Cryotop® method is simple, safe, universal and easy to apply for everyone.

The composition of the Kitazato media is entirely synthetic. Among the components, the following are notable:

  • HPC – Hydroxypropyl Cellulose Prevents the risk of contamination, increases the rate of survival in hatched blastocysts and reduces mechanical stress during warming.
  • Trehalose Functions as an osmotic agent in place of sucrose; provides greater safety in the process, improving the protection of the cellular membranes.

The VT801 vitrification media allow up to 5 procedures to be carried out and consist of:

  • 1 x 1.5ml Vial BS (Basic Solution).
  • 1 x 1.5ml Vial ES (Equilibrated Solution).
  • 2 x 1.5ml Vials VS (Vitrification solution).

 

The VT802 warming media allow up to four procedures to be carried out and consist of:

  • 2 x 4ml Vials TS (Thawing Solution).
  • 1 x 4ml Vials DS (Dilution Solution).
  • 1 x 4ml Vial WS (Washing Solution).

All Kitazato media must be kept between 2ºC and 8ºC, out of sunlight.

Highest survival rates

Cryotop® Method offers proof of its outstanding results. Our suvival rates over 90% are backed up by numerous clinical studies with the biggest sample sizes in the market.

  • 100%
  • 90%
  • 80%
  • 70%
  • 60%
  • 50%
  • 40%
  • 30%
  • 20%
  • 10%
  • 0%
  • Oocytesn=3.286
    Cobo
    2010
  • Oocytesn=124
    Rienzi
    2010
  • Oocytesn=168
    Parmegiani
    2011
  • Oocytesn=898
    Solé
    2013
  • Embryosn=124
    Rienzi
    2012
  • Embryosn=168
    Parmegiani
    2012
  • Embryosn=898
    Solé
    2013
  • Blastocystsn=168
    Parmegiani
    2011
  • Blastocystsn=898
    Solé
    2012
Cryotop® Survival rates in human specimen
  • pH: 7.2 - 7.6
  • Osmolarity
  • Endotoxin: <0.25 EU/mL by LAL methodology
  • Sterility (Bacteria, Fungi): current USP Sterility Test <71>
  • MEA (Mouse Embryo Assay): One Cell Assay >80% after 96 hours

Instructions

ORDER NUMBER CODE DESCRIPTION
91101 VT601 Vitrification Solutions *
91121 VT602 Thawing Solutions *
91171 VT801 Vitrification Solutions
91182 VT802 Thawing Solutions
81115 CR-Y Cryotop® Yellow
81114 CR-B Cryotop® Blue
81113 CR-W Cryotop® White
81112 CR-R Cryotop® Red
81111 CR-G Cryotop® Green
K Reproplate
94123 SET RACK-L Cooling Rack+Steel Box-Large
94122 SET RACK-S Cooling Rack+Steel Box-Short
81125 CR SC-Y Cryotop® SC Yellow
81124 CR SC-B Cryotop® SC Blue
81123 CR SC-W Cryotop® SC White
81122 CR SC-R Cryotop® SC Red
81121 CR SC-G Cryotop® SC Green
84115 BLOCK SC Aluminum Block for Cryotop® SC
84117 CUTTER SC Straw Cutter for Cryotop® SC
84119 HEAT SEALER Heat Sealer for Cryotop® SC
*These products are only available in selected countries

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