Vitrification and Warming Media

Kitazato media are the most versatile option for cryopreservation in your laboratory. Reduce your costs by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from zygote stage to blastocyst.

Cryopreservation solution - Media for vitrification and warming of oocytes and embryos

The revolutionary device by Kitazato has been applied in over 1,500,000 clinical cases for oocytes and embryos with this method of cryopreservation during the last decade, obtaining excellent results in over 90 countries and 2,200 assisted reproduction centres. The Cryotop® method is simple, safe, universal and easy to apply for everyone.

The composition of the Kitazato media is entirely synthetic. Among the components, the following are notable:

Hydroxypropyl cellulose (HPC) prevents the risk of contamination, increases the rate of survival in hatched blastocysts and reduces mechanical stress during warming.
Trehalose functions as an osmotic agent in place of sucrose. It provides greater safety in the process, improving the protection of the cellular membranes.
The incorporation of gentamicin prolongs the sterility of the media, guaranteeing greater safety in handling the solutions.

The VT601 Vitrification media allow up to 5 procedures to be carried out and consist of:

1.5 ml Basic Solution vial (BS)
1.5 ml Equilibrated Solution vial (ES)
2 x 1.5 ml Vitrification Solution vial (VS)

The VT602 Thawing media allow up to 4 procedures to be carried out and consist of:

2 x 4 ml Thawing Solution vial (TS)
4 ml Diluent Solution vial (DS)
4 ml Washing Solution vial (WS)

All vitrification media must be kept between 2ºC and 8ºC.

VT601 and VT602 have a shelf life of 12 months.

VT801 and VT802 available in selected countries.

Highest survival rates

Cryotop® Method offers excellent survival rates for oocytes and embryos in all stages of development, reported in numerous clinical publications with the largest study samples in the whole sector

Cryotop^® Survival rates in human specimen

100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%

Oocytesn=2.770
Braga D.P.
2016
Oocytesn=591
Chamayou S.
2017
Oocytesn=35.654
Cornet-Bartolomé D.
2020
Embryosn=303
Xue Y.
2018
Embryosn=2.617
La Marca A.
2019
Blastocystn=528
Coello A.
2017
Blastocystn=8.736
Ueno S.
2018
Blastocystn=3818
Hernández-Nieto et al.
2019
PGT Embryosn=90
Sekhon L.
2018
PGT Embryosn=2.129
Cimadomo D.
2018

In our publications section you will find the most relevant research on vitrification conducted using Kitazato products by IVF professionals and scientists from all over the world.

ORDER NUMBER	CODE	DESCRIPTION
91101	VT601	Vitrification Solutions *
91121	VT602	Thawing Solutions *
91171	VT801	Vitrification Solutions *
91182	VT802	Thawing Solutions *
81115	CR-Y	Cryotop® Yellow
81114	CR-B	Cryotop® Blue
81113	CR-W	Cryotop® White
81112	CR-R	Cryotop® Red
81111	CR-G	Cryotop® Green
	K	Reproplate
94123	SET RACK-L	Cooling Rack+Steel Box-Large
94122	SET RACK-S	Cooling Rack+Steel Box-Short
81125	CR SC-Y	Cryotop® SC Yellow
81124	CR SC-B	Cryotop® SC Blue
81123	CR SC-W	Cryotop® SC White
81122	CR SC-R	Cryotop® SC Red
81121	CR SC-G	Cryotop® SC Green
84115	BLOCK SC	Aluminum Block for Cryotop® SC
84117	CUTTER SC	Straw Cutter for Cryotop® SC
84119	HEAT SEALER	Heat Sealer for Cryotop® SC

Vitrification and Warming Media

The composition of the Kitazato media is entirely synthetic. Among the components, the following are notable:

The VT601 Vitrification media allow up to 5 procedures to be carried out and consist of:

The VT602 Thawing media allow up to 4 procedures to be carried out and consist of:

Highest survival rates

Instructions