ContactVitrification and Warming Media
Kitazato media are the most versatile option for cryopreservation in your laboratory. Reduce your costs by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from Pronucleus to Blastocyst.
The revolutionary device by Kitazato has been applied in over 1,500,000 clinical cases for oocytes and embryos with this method of cryopreservation during the last decade, obtaining excellent results in over 90 countries and 2,200 assisted reproduction centres. The Cryotop® method is simple, safe, universal and easy to apply for everyone.
The composition of the Kitazato media is entirely synthetic. Among the components, the following are notable:
- HPC – Hydroxypropyl Cellulose Prevents the risk of contamination, increases the rate of survival in hatched blastocysts and reduces mechanical stress during warming.
- Trehalose Functions as an osmotic agent in place of sucrose; provides greater safety in the process, improving the protection of the cellular membranes.
The VT801 vitrification media allow up to 5 procedures to be carried out and consist of:
- 1 x 1.5ml Vial BS (Basic Solution).
- 1 x 1.5ml Vial ES (Equilibrated Solution).
- 2 x 1.5ml Vials VS (Vitrification solution).
The VT802 warming media allow up to four procedures to be carried out and consist of:
- 2 x 4ml Vials TS (Thawing Solution).
- 1 x 4ml Vials DS (Dilution Solution).
- 1 x 4ml Vial WS (Washing Solution).
All Kitazato media must be kept between 2ºC and 8ºC.
Highest survival rates
Cryotop® Method offers proof of its outstanding results. Our suvival rates over 90% are backed up by numerous clinical studies with the biggest sample sizes in the market.
- 100%
- 90%
- 80%
- 70%
- 60%
- 50%
- 40%
- 30%
- 20%
- 10%
- 0%
- Oocytesn=3.286
Cobo
2010 - Oocytesn=124
Rienzi
2010 - Oocytesn=168
Parmegiani
2011 - Oocytesn=898
Solé
2013 - Embryosn=124
Rienzi
2012 - Embryosn=168
Parmegiani
2012 - Embryosn=898
Solé
2013 - Blastocystsn=168
Parmegiani
2011 - Blastocystsn=898
Solé
2012
Cryotop® Survival rates in human specimen
- pH: 7.2 - 7.6
- Osmolarity
- Endotoxin: <0.25 EU/mL by LAL methodology
- Sterility (Bacteria, Fungi): current USP Sterility Test <71>
- MEA (Mouse Embryo Assay): One Cell Assay >80% after 96 hours
Instructions
| ORDER NUMBER | CODE | DESCRIPTION |
| 91101 | VT601 | Vitrification Solutions * |
| 91121 | VT602 | Thawing Solutions * |
| 91171 | VT801 | Vitrification Solutions |
| 91182 | VT802 | Thawing Solutions |
| 81115 | CR-Y | Cryotop® Yellow |
| 81114 | CR-B | Cryotop® Blue |
| 81113 | CR-W | Cryotop® White |
| 81112 | CR-R | Cryotop® Red |
| 81111 | CR-G | Cryotop® Green |
| K | Reproplate | |
| 94123 | SET RACK-L | Cooling Rack+Steel Box-Large |
| 94122 | SET RACK-S | Cooling Rack+Steel Box-Short |
| 81125 | CR SC-Y | Cryotop® SC Yellow |
| 81124 | CR SC-B | Cryotop® SC Blue |
| 81123 | CR SC-W | Cryotop® SC White |
| 81122 | CR SC-R | Cryotop® SC Red |
| 81121 | CR SC-G | Cryotop® SC Green |
| 84115 | BLOCK SC | Aluminum Block for Cryotop® SC |
| 84117 | CUTTER SC | Straw Cutter for Cryotop® SC |
| 84119 | HEAT SEALER | Heat Sealer for Cryotop® SC |
